Science finally agreed with me!

I don’t even know how to even start with this last blog post. I learned so many things in this REU! Namely, I learned about the immense amount of thought that has to go into writing methods; every single little detail matters so much more than you initially thought it did. It took me like four tries to figure that out (five if you count my… experience with the ctenophores). Another important thing I learned was all the interesting careers and opportunities outside of academia and research. And all this time I’ve been thinking research was all that there was (thank goodness it’s not). All in all, it has been an absolutely beautiful and amazing summer filled with people I can truly say I will never forget. We had so many laughs and adventures and unexpected moments, it’s gonna be really, really hard to say goodbye to all of them tomorrow. Rest assured there will be plenty of waterworks. I’m gonna miss the MSI so much, I’m gonna miss Bella, the science dog so much, but most of all I’m gonna miss my REU fam! To finish my very last blog post here is my (modified) abstract:

Phytoplankton play a key ecological role as the base of the marine food web and as regulators of atmospheric carbon. However, little is known about the ways in which these organisms interact with petroleum-related compounds. In the face of this growing environmental problem, insights into phytoplankton-petroleum interactions has never been more important. In this study, we exposed Pyrocystis noctiluca, an open ocean dinoflagellate, to varying levels of crude oil, dispersant, and chemically dispersed oil, and evaluated its response in terms of growth rate and individual

20160802_122150

The fam!!!!!! 

bioluminescent output. Given the toxicity of crude oil and dispersant, and its known effects on other plankton species, we hypothesized P. noctiluca to exhibit a decrease in growth rate and bioluminescent output as crude oil and dispersant concentrations increase. Additionally, we expected the dispersed oil treatment to have the strongest effect on P. noctiluca’s growth and bioluminescence. Our results show that crude oil in the form of the water accommodated fraction and dispersed oil both have an inhibitory effect on the growth of P. noctiluca, while dispersant only has an effect at extremely high concentrations. Bioluminescence was affected only by dispersed oil, which suggests that dispersed oil may affect the biochemistry of bioluminescence. We conclude that P. noctiluca is resistant to typical levels of dispersant used in the aftermath of an oil spill in both its growth and bioluminescence. However, our results suggest that this species is vulnerable to petroleum pollution at levels commonly found in the water column after a spill. Due to the threat that oil pollution presents and the importance of phytoplankton to marine ecosystems, it is imperative that we continue studying phytoplankton-oil interactions and effects.

Exito a todos!

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All summer 16, including sediment characteristics of the tidal freshwater zone!

lib group picThis summer has been a truly amazing learning experience that I will remember and cherish for years to come. I have met so many wonderful people at UTMSI and had the incredible opportunity to make great connections and friendships. I am sad today is my last day in Port Aransas, I will miss this campus, the staff, my mentors and fellow lab members and last but not least I will miss my REU family. I love you guys, thank you for all the amazing memories, without you this summer would have not been the same at all.

reu group picI learned so much in Amber Hardison and Jim McClelland’s lab this summer while working on my research project. With the help of my mentors and the grad students in my lab I successfully finished this summer by presenting my research. Below is a copy of the abstract used for my presentation in the REU research symposium: a summary of my research project and my findings. Cheers!

Tidal freshwater zones (TFZ) are understudied areas of rivers that experience tidal variations that swing between river and reservoir-like conditions. The TFZ often feature long residence time, slow flow rates, and even reverse flow directions. Such unique hydrodynamic conditions can affect nitrogen cycling within the zone profoundly, with possibly enhanced nitrogen transformation and removal in the interface between sediment and water. A lot of nitrogen cycling pathways take place in the sediment/water interface such as denitrification and remineralization. Thus, the physical characteristics of sediment, such as grain size distribution and porosity, may influence the organic content (OC) of sediments, which affect the cycling of nitrogen in the river. To understand the role of sediment-water processes in the transformation and removal of nitrogen in the TFZ, we 1) quantified grain size and porosity of sediments at five study sites along the TFZ in the Aransas and Mission rivers; 2) investigated how the physical characteristics of sediment could affect OC of sediments; 3) determined how OC varies spatially and seasonally within the TFZ.

group pic reu w deanaOur findings show the different geological characteristics in terms of grain size and porosity between both rivers. The sediments of the Mission get finer downstream. Carbon and nitrogen content follow similar pattern of grain size and porosity in both rivers. There is a strong correlation between the physical characteristics of the sediments and the organic content in the rivers. Mission has higher OC than Aransas, probably due to its higher sediment porosity. There is considerable spatial variability in the Aransas river. No obvious seasonal patterns of OC were found in the rivers. Overall, my result points out that physical characteristics of sediment have strong influence on OC which is important for respiration of microorganism communities. The data and the results of this study will be compiled with future research and thus provide a better understanding of the sediment/water interface and the nutrient cycling that occurs in the TFZ.

 

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My FIN-ished Summer Research Project

The last ten weeks of summer has passed by within a blink of an eye, it’s hard to believe that I’ll be leaving in the morning. This REU has been a wonderful experience and has taught me so much about research in marine science. In just these few short weeks, I got to explore a wide number of ways to study and research fisheries that I know I can use throughout my career. In addition, I got to meet so many amazing people who I’m so glad to call my coworkers, friends, and family. No matter where our careers take us, I know we will remain close and I can’t wait to see how successful everyone will be in the future!

Group Pic

Since my last blog post, my project has changed a little bit to accommodate my results. Below is my abstract that describes my new project and a summary of what I found. In addition, below are the two maps I created of the distributions of Spotted Seatrout and Silver Perch in relation to the size of their spawning aggregations and habitat type.

The fish family Sciaenidae has developed a unique adaptation of vocalizing to communicate during the process of reproduction. Two species of fish in this family are present in the estuaries surrounding Port Aransas, Texas—Spotted Seatrout (Cynoscion nebulosus) and Silver Perch (Bairdiella chrysoura). In these species, the male fish make vocalizations when they are actively spawning, releasing their sperm into the water column. In addition, both Silver Perch and Spotted Seatrout reproduce in spawning aggregations, or a dense gathering of fish that occurs when males and females alike are actively spawning at one time. These sites are important to study in particular because they are how most of the spawning in these fish species occurs and are vital to their reproductive success. We used passive acoustic technology to survey the surrounding bays of Port Aransas for spawning aggregations of Spotted Seatrout and Silver Perch. Once the fish were identified and quantified, I mapped the distribution of aggregations found during our sampling. I analyzed whether temperature, depth, salinity, and habitat type affect the presence of spawning aggregations for each species, as well as aggregations where both are present. Results found that temperature does affect the presence of spawning aggregations in both species and salinity was also found to have an effect on the likelihood of Spotted Seatrout and Silver Perch spawning together instead of in single-species aggregations. This study gives a greater understanding of where and how these two species of fish reproduce, which is important for ecological and conservation needs.

Silver Perch MapSpotted Seatrout Map

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Diatoms are great

 

Abstract time: Structures for motility are non-existent in vegetative-stage centric diatoms yet they exhibit a sinking property and have been found to be positively buoyant. The sinking and ascent rates of diatoms can vary for different reasons within species as well as between species. This could allow them to migrate tCapturedo areas abundant in nutrients and light which is key to their survival. These rates can have a large impact by affecting the transport of carbon to the ocean floor. To obtain these rates, the following methods were used: the SETCOL method, where the rates are a measure of a homogenous population, and particle tracking via video recording, where the rate of each individual particle is measured. To study the differences in mean sinking and ascent rate outputs of both methods, a model incorporating the basic principles of each was created using Python 2.7. The model will prove useful in determining combinations of sinking and ascent rmeates that lead to a positively buoyant SETCOL measurement. We will also be able to assess how individual sinking rates modify the population’s mean sinking rate and the percent of buoyant particles that must be present in a population for there to be a significant mean ascent rate measurement.

So this is the last blog post. It’s been an amazing experience with amazing people. I learned so much from my mentors and I really appreciate the time they spent teaching me. I also want to thank them for turning me into a diatom believer. This summer would not have been complete without my REU family. They’re the best bunch of people. I’ll definitely miss it all and look forward to our REUnion.

Posted in student projects

Final blog post

20160729_113234Well everyone, this is it, my final blog post for the summer.  I’ve learned a lot and spent some great moments here at UTMSI and I’d like to thank everyone that made it possible.  Our mentors provided us with useful advice and lessons that will definitely help us in our future years.  And finally my fellow REUs made the entire experience that much greater, we had many laughs and moments we’ll never forget.  It was definitely an unforgetable experience from which we all learned a lot, and I’m sure going to miss everyone. But as is the custom, here is the abstract for my seminar presentation.

 

In recent years there has been a large expansion of the tree of life from the massive number of novel genomes that have been obtained using metagenomics in recent years. Examination of commonly used amplification primers for 16S rRNA gene diversity surveys revealed that they overlook many newly discovered archaeal groups. Thus, we redesigned universal PCR primers 530F and 907R that target novel archaeal groups. The community structure of the Mission Aransas NERR Research Reserve (near Port Aransas, TX) shallow estuary sediments has not been previously explored.  To investigate the diversity of these communities we sectioned (3 cm intervals, down to 15 cm) sediment cores from 3 different sites (east and west Copano Bay and Mesquite Bay) in the reserve.  Dissolved oxygen, nitrate, and nitrite porewater concentrations were measured in the sediments and the cores were subdivided to extract total DNA and RNA.  Phylogenetic analyses revealed the presence of several novel lineages including 4 archaeal phyla and 9 different bacterial phyla in Copano Bay. The archaea belong to phyla that have been previously associated with marine and estuary sediments around the world including 2 clades (Altiarchaeales and a novel lineage) within the DPANN superphylum, Euryarchaeota (Marine Benthic Group D), and Bathyarcheota. The bacteria include Planctomycetes, Betaproteobacteria, Deltaproteobacteria, and candidate phyla WS3 and OP8, and what appears to be a novel phylum.  This novel phylum is related to sequences previously recovered from South Pacific deep abyssal sediments. While these new primers revealed there is substantial novel diversity present in the NERR reserve, much work remains to be done involving the geochemical properties of the sediments and the ecological roles of these organisms.

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Epic Summer, New Experiences

Wow! It’s hard to believe that it’s already the last day of the REU! At first I was nervous, but I am more than glad I accepted this adventure. During the summer I had the amazing opportunity to work under the mentoring of Deana Erdner and wow! I learned a lot! When starting with my experiment I was a bit worried, to be honest, qPCR was something that I had never heard before. Thankfully Deana, and her lab (Tatiana, Ingrid, Yida), where always there for me. In terms of my project, thinks turned out really good! For my project I wanted to see if there was a change in the rRNA gene content of the A. tamarense during different growth phases, and after dealing with the qPCR and the standards, my results demonstrated a variability in the gene content. My experiment changed a lot from what I started with on June,  so to keep you updated, here is the abstract for my REU-experiment during this summer 2016.

Ribosomal RNA gene content of the dinoflagellate Alexandrium tamarense

Phytoplankton are planktonic phototrophic organisms that play an important role in the marine ecosystem. They grow according to the availability of carbon dioxide, sunlight and nutrients. It is often challenging to understand them in their natural environment, for why scientists have been studying the ribosomal RNA (rRNA) to understand more about their physiology and way of living.  The rRNA is essential to the cell because it provides structure for ribosomal protein. There have been published and unpublished studies demonstrating that there have been cases when the rRNA gene content from phytoplankton changes. For this project, quantitative PCR (qPCR) was used to analyze the rRNA gene content of the phytoplankton dinoflagellate Alexandrium tamarense. For the qPCR, the fluorescence DNA-binding dye SYBR green assays was used. The gene content was analyzed from two different perspectives: 1. taking samples in different growth stages; 2. exposing cultures to different amount of light to see if there is a relation between growth rate and rRNA gene copy number. The growth stages used for the first experiment were early and late exponential. For the second experiment, cultures were grown in different amount of light and synchronized to  receive an accurate copy per cell number. The results show an unexpected change in rRNA gene content during the two phases of the early and late exponential stage. The numbers increased from early exponential to late exponential. The results were contradict for  what has been seen in previous published studies. Additionally, a variability in rRNA gene content was observed from the different levels of light. This bring us to the possibility that the change in gene copy number is related to physiological adaptations.

I’m leaving Port AranIMG_0357 (1)sas Tx. with many good memories. Besides learning new things everyday, and having an amazing mentor, I also met a lot of amazing people it the UT-MSI. Specially the other REU students (fam). If it wasn’t because of them, their support, and their craziness, this summer experience would have not been the same! I am really grateful for this amazing opportunity, and I know this is the beginning to a new journey.

 

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Final post

A lot of data has been gathered about lentic ecosystems, such as lakes and ponds, and lotic ecosystems, such as rivers. However, there is a gap in the literature that doesn’t address ecosystems that fall somewhere in between. One example of this is in the tidal freshwater zone. Much of the time the tidal freshwater zone flows like a river, however, when the tide rises it can cause the flow of the river to stop completely and in some cases can even cause the flow to reverse. For my project, I specifically focused on sediment respiration. Most reactions happen where the sediment meets the water so the tidal freshwater zone cannot be fully understood without a full understanding of the processes occurring along this boundary.

My goal was to determine how respiration rates differ in various tidal freshwater zones of each river. In addition, I compared respiration rates in winter months to summer months. To estimate respiration rates, I measured oxygen consumption rates and porewater nutrient concentrations at these locations. I used an oxygen microsensor to create a profile of the sediment because oxygen is required for aerobic respiration and, therefore, it is a direct indicator of cellular respiration. I also analyzed the porewater to determine fluctuations in porewater nutrient concentration since these nutrients are a product of decomposition. My findings show that respiration rates increase linearly as you move downstream. In addition, I found that respiration rates were higher in summer months. When analyzing my porewater, I found a similar bell shaped curve showing ammonium concentrations to be highest in the center of the tidal freshwater zone on both rivers. The biggest factor I found that effected respiration rate was the sediment size, the smaller the sediment size, the higher the rate of respiration.

My findings were significant because they insinuate that the tidal freshwater zone is effective and efficient at removing organic matter from the water column. This is important because if the organic matter is not removed it will flow into the estuaries and tidal waters where it will promote the growth of phytoplankton and algae and eventually alter the ecosystems in these areas.G and i with core

I learned more this summer than I ever could have imagined. All of the members of my lab were so patient and easy to work for. I was also very lucky to have such a fun group of people to go through my REU with. I look forward to watching my project progress with the next sampling series. A huge thanks to everyone, hasta luego!

Posted in student projects

An awesome summer of research

Hi everyone, this is the final blog post that I’ll be posting for this REU. All in all, I had a great summer of research and learned a lot, and met a lot of great people (especially my REU fam that I became so close with). Below is the abstract for my summer research symposium that I created.

Freshwater mussel species Quadrula. aurea and Quadrula. petrina have shown a strong decline in population density in Texas during recent years for unknown reasons (Burlakova et al., 2011). Sclerochronology, the study of growth patterns in hard tissues of animals, could help to identify the underlying cause of the decline by reconstructing historical population-wide growth patterns. Such growth patterns can be integrated with climate data to establish the drivers of growth. When using this approach, it is crucial to correctly identify annual increments in the prismatic layer of the mussel shells. Stable isotopes of oxygen (δ 18 O) and carbon (δ 13 C) were used to assess if visually identified increment boundaries on the mussel shells represented annual growth rings. This was done by checking for consistent oscillations of isotope values between growth boundaries. Oscillations in the isotope values likely reflect seasonal climate variation, with previous studies showing higher δ 18 O and δ 13 C values during winter (Versteegh et al., 2011). In this experiment, a computer operated micromill was used to extract carbonate samples along the prismatic growth axis in the shells of five mussel specimens for each species in order to be to be analyzed for their δ 18 O and δ 13 C values. Optimal sample weights were within 170-210µg. A significant correlation was found between δ 18 O and δ 13 C with Q. aurea(r=.69, p=7.85×10 -9 ) suggesting that climate drivers affect δ 18 O and δ 13 C variability alike. However, Q. petrina did not exhibit this correlation. The results showed that the seasonality of the isotopes did not match well with visually identified growth increments on the shell, indicating these might not represent annual growth increments. However, when averaging isotope values within visually identified ring boundaries (and in doing so, assigning a specific calendar year to these isotope values), weak trends were found between temperature and carbon and oxygen isotope values, which is consistent with previously published results (Dettman et al., 1998). The weakness of the trends is possibly due to a small sample size, unclear growth boundaries, boundaries, and the use of air instead of water temperature. From these results, it seems that these species seem to have low potential for use in sclerochronology; however, this shortcoming may be overcome with older specimens and a larger sample size.

 

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Isolating Single Cells from Phytoplankton

The Flow Cytometer I was going to use

The Flow Cytometer

One of the normal things people say about science, is that things almost never goes by the way you expect them to, that behind every project there are many backup plans, and I’ve proven this to be right. For the last few weeks I have been growing cultures of six different phytoplankton, and running fluorescence test on each one of them to keep track of their growth phase: exponential or stationary. With the intention of isolating single cells from each culture and do the qPCR and cell cycle analysis. This way I would be able to know the rRNA gene content number. Plan A was to isolate single cells using the flow cytometer: a machine that analyzes single cells, making them pass through a laser and using fluorescence signals to sort the cells and separate them into different properties using light.

So many Alexandium cells moving around!

So many Alexandrium cells moving around!

Still, the flow cytometer wouldn’t work when I tried to use it. Therefore I had to go with plan B: isolate singles cells from one of my phytoplankton cultures with a pipette. Meaning, I spent a day, in front of a microscope, isolating 78 single cells from Alexandrium (the phytoplankton I ended up choosing). After isolating the cells, I ran qPCR analysis on them. This was made today, and it appears to be successful, still the data needs yet to be analyzed to see how it turned out. Hopefully it will turn out the way I expect it to, and even better, we manage to fix the flow cytometer.

 

There is ONE cell in each of this PCR's wells!

There is ONE cell in each of this PCR’s wells!

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Captain’s Log: Update on Phytoplankton Research

Hello everybody! It has now been about a month living in Port Aransas and working in Dr. Zhanfei Liu’s lab. I must say that I am having the greatest summer of my life here. I’ve met and became great friends with the other interns, faculty, and graduate advisers. The atmosphere here is very positive and I feel like I can accomplish any goals and milestones on my project and potential career.

I have now developed a protocol for what I want to do with my research. My overarching question is, “Is there a definite spatial and temporal distribution of phytoplankton and nutrients between the San Antonio, Lavaca, Mission-Aransas, and Nueces rivers?”. My hypothesis is that distribution of phytoplankton and nutrients increases at lower latitudes. There have already been major weather events such as flooding earlier this year. Each river may vary in nutrient and pigment concentrations after this weather event due to the size and spread of their corresponding watersheds. Furthermore, I predict that the San Antonio river will have the greatest amount of diversity and nutrient concentration within its community structure due to its enormous length and watershed size encompassing many towns and cities. I also predict that pigment composition will be its highest in all rivers during the summer season.

 

Extraction of pigments from GF/F filters using acetone

Extraction of pigments from GF/F filters using acetone

Extracts are loaded in this sample holder.

Extracts are loaded in this sample holder.

Analysis of pigment types and concentrations are achieved through instrumental analysis via HPLC

  Analysis of pigment types and concentrations are achieved through instrumental analysis via HPLC

Peaks on the data spreadsheet indicate a certain pigment and its relative concentration

Peaks on the data spreadsheet indicate a certain pigment and its relative concentration

Science!

Science!

Summer 2016 Phytoplankton community profiles will be created via HPLC analyzed pigment composition of the river samples.  These will then be graphed and coupled with analyzed data from fall 2015, winter 2016, and spring 2016 to determine a pattern, if any, between seasons, latitude, and nutrient concentrations of the five rivers.  Diversity and dominant species of phytoplankton communities will be calculated using Shannon-Weiner Diversity Index and Simpson’s Dominance Index.  Similarity between the five rivers will be analyzed using Bray-Curtis Index.

I have already analyzed pigment samples from fall 2015 to spring 2016. My next task is to go and sample the five rivers within the first week of July. After all nutrient and pigment samples have been ran, I will then process and integrate the metadata! I’m so excited about the this research and I think I will find something pretty interesting about these rivers! Thanks for reading and stay tuned for future blog posts! 🙂

 

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