Hi everyone, we are already on week 5 and I’ve just obtained my samples from the NERR today, which means next week is going to be filled with DNA and RNA extractions and maybe even a PCR or two. Also I’ve been hard at work extracting DNA and RNA from the samples sent from the White Oak River estuary and running PCR reactions with 16S primers from Asgard and Archaea to see if I can find any Asgard archaea in them. So far, through trial and error I have been able to obtain one product with a high possibility of being from Asgard and am currently on the process of cloning the reaction into E. coli in order to isolate the 16S gene I need.
Analyzing samples from the White Oak River estuary and the NERR
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