Buoyancy in Diatoms Project Update


Hi people, we have decided to look on how the light intensity affects diatoms sinking and ascent rates. I am already growing cultures of Thalassiosira spp., Pseudonitchzia spp., and Chaetoceros spp. in four different light intensity levels (150, 75, 25, and 18 µE/m2s). My next step will be adding new cultures of Odontella spp., Skeletonema spp., and Rhizosolenia spp. to the treatments that I had already mentioned. Also, we are looking to grow additional cultures with high salinity medium because the local sea water salinity decreased. By doing this we have a backup in case that the diatoms do not grow well in low salinity medium. Preliminary data from a control run of Thalassiosira spp. recording  shows that their average velocity of sinking is approximately 0.2 meters per day. I observed one Thalassiosira spp. cell ascending,which represents 2% of the selected trajectories. This data suggests that we may have to increase the diatom density to see more positive buoyancy.

 

Odontella

Odontella spp.

Thalassiosira spp. (From Google Images)

Thalassiosira spp. (From Google Images)

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Incubator(to keep cultures alive)

Graph

Thalassiosira spp. data

Chaetoceros spp

Chaetoceros spp.

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Instrument for light measurement

Pseudonitzchia

Pseudonitzchia spp. (From Google Images)

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