Hi people, we have decided to look on how the light intensity affects diatoms sinking and ascent rates. I am already growing cultures of Thalassiosira spp., Pseudonitchzia spp., and Chaetoceros spp. in four different light intensity levels (150, 75, 25, and 18 µE/m2s). My next step will be adding new cultures of Odontella spp., Skeletonema spp., and Rhizosolenia spp. to the treatments that I had already mentioned. Also, we are looking to grow additional cultures with high salinity medium because the local sea water salinity decreased. By doing this we have a backup in case that the diatoms do not grow well in low salinity medium. Preliminary data from a control run of Thalassiosira spp. recording shows that their average velocity of sinking is approximately 0.2 meters per day. I observed one Thalassiosira spp. cell ascending,which represents 2% of the selected trajectories. This data suggests that we may have to increase the diatom density to see more positive buoyancy.